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A portion of brain tissue (100 mg) was taken in a 900-microliter volume of PBS (1:9 ratio) in a glass homogenizer on ice and homogenized at 2,000 rpm for 5 min

This effect is particularly relevant in metabolic research contexts where metabolic imbalance contributes to hyperglycemia [6] : Mechanism of Glucose Control The exact mechanisms remain under investigation, but evidence suggests: Direct effects on pancreatic cells via local amylin receptor activation Indirect modulation through delayed nutrient absorption (slower gastric emptying) Central nervous system-mediated effects on metabolic regulation [6] By reducing hepatic glucose output during the postprandial period, cagrilintide decreases glucose production, contributing to improved glycemic control independent of its effects on insulin secretion [6]

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Thus, sleep deprivation may impair memory consolidation in part by reducing the synthesis of proteins needed to support synaptic plasticity [31,32]

The concept of the gut-brain-ear axis has emerged, linking imbalances in the gut microbiota (dysbiosis) to the development of hearing loss 38 , exposure to environmental noise stress 39 , and chronic tinnitus 28