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Unlike the potent TGR5 agonists, DCA and LCA, we could not detect any effects of TMCA on cAMP levels in the STC-1 cells, NCI-H716 cells, and mouse intestinal organoids, suggesting that the increased ileal TMCA observed in the mice after T3 treatment might not act via TGR5-mediated pathway

if dietary patterns remain unchanged, weight loss and glycaemic benefits will be limited

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Kelley added that other issues, including the duration of medication availability through TrumpRx, need to be resolved

After incubating with HRP-conjugated secondary antibody (at 1 : 10 000 dilution), the proteins were visualized by using an ECL kit and ImageQuant LAS 4000mini (GE Healthcare)