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Energy minimization was performed in two stages: first with restraints on the protein-ligand complex (500 steps steepest descent followed by 2500 steps conjugate gradient), then without restraints (1000 steps steepest descent followed by 4000 steps conjugate gradient)

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Linthorst et al., 1989), they are often used as markers for SA-mediated resistance in the early stages of viral pathogenesis (van Loon et al., 2006

The average time of back pain evolution was 10 years