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Due to the parallel orientation of both electrons (electron spin S = 1), molecular oxygen is sometimes referred to as triplet oxygen (multiplicity 2 S + 1 = 2 1 + 1 = 3) and is denoted as 3 O 2 (Halliwell and Gutteridge 2015)

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The dose of both donor plasmid and Cas9 plasmid was 0.5 g g 1 , with an injection volume of 10 l g 1 for all treatment groups

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