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Total RNA isolation, cDNA synthesis, and qRT-PCR TransZol RNA kit (ET101-01, TransGen Biotech) was used to extract total RNA from Arabidopsis plants according to manufacturers instructions

Typical subject profiles for each quartile of MACE risk are reported in Additional file 7: Appendix Table S6)

Furthermore, enhanced permeability of the blood-brain barrier is a critical neurovascular complication of T2DM, which disrupts CNS homeostasis and impairs its function