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Mast Cells stimulate the release of histamine, leukotrienes and cytokines which result in the characteristic allergic symptoms

Arch Gynecol Obstet (2008) 277(6):53946

Erythrocyte Cell Lysis Assay The CB compounds identified as lead compounds were analyzed at 10 serial dilutions using fresh mouse erythrocytes at 1% hematocrit in Dulbeccos PBS (DPBS, Gibco ) in V-bottom microplates (Corning 96 well TC-treated microplate)