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Intestinal tissue segments were homogenized, lysed (1 M Tris-HCl, 5 M NaCl, 1% (v/v) Igepal CA-630, 0.5% (w/v) sodium deoxycholate monohydrate, and one tablet of EDTA-free protease inhibitor cocktail), and centrifuged at 10,000 x g for 10 min to prepare the tissue extracts