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ROS quantification was performed on a Spark Multimode Microplate Reader (Tecan Life Sciences, Mannedorf, Switzerland) at an Ex/Em of 485/535 hourly for 8 h

Because your body already produces it, its generally well tolerated when administered properly

The major isoform, 23 kDa prolactin, acts via its membrane receptor, the prolactin receptor (PRL-R), which is a member of the haematopoietic cytokine superfamily and for which the mechanism of activation has been deciphered

In addition to this, the nature of your deficiency, and level of deficiency is typically not going to require such a high dosage at once