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The authors would like to thank Ms Nakai (Nara Medical University) for assistance in collecting the clinical data

Adequate protein intake is important to preserve lean muscle mass during weight loss, but specific requirements should be discussed with your healthcare team, especially if you have kidney disease or other health conditions

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The monolayer was scratched with a sterile 200 L pipette tip, and cells were then treated with the TGF-1 (10 ng/ml) + IL-1 (10 ng/ml) for 24 h at 37 C