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They disappeared because the machinery moved slowly and patients couldn't wait

Large cgMD simulations including increased numbers of WT or mutant receptors in a membrane environment with varying cholesterol levels could also be performed to determine the effect of cholesterol on GLP-1R oligomerisation

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To determine if GLP-1R induces cAMP generation specifically at VAPB locations, we modified a fluorescence resonance energy transfer (FRET)-based system of biosensors targeted to endogenous proteins (FluoSTEPs) 25 , so that a FRET cAMP sensor would fully reconstitute only in the presence of the GFP 11 fragment fused to VAPB, resulting in functional biosensor assembly specifically at VAPB loci (Supplementary Fig

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