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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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Particularly in vascular regulation, activation of 1-AR causes vasoconstriction and increases blood pressure, while activation of 2-AR induces vasodilation and lowers blood pressure 5,109,110,111

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